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    Poster

    Blood Transfusions in Cynomolgus and Rhesus Monkeys: A Retrospective Analysis Over a 20-Year Period (P427)

    Monday, November 18, 2024
    5:00 PM - 6:30 PM CT
    An increasing number of cell therapies require myelo-conditioning to increase cell engraftment. Antibiotics and blood transfusions are the main supportive care to mitigate pancytopenia following myelosuppression.
    Platelet (PLT) characteristics have a 10-day maturation period, a ~4.5 day half-life, a lifespan of 8-11 days, and ~60-75% PLT are present in circulation (with remaining PLT mostly in the spleen) . Transfused PLT have shortened half-life (2.6 days).
    A retrospective analysis of blood transfusion data in non-human primates over a 20-year period at a single research facility was conducted. An important benefit from transfusion is a proportional increase in PLT, red blood cells (RBC) and hematocrit (HCT).
    The number of transfusions (6-13 per animal), the time between required transfusions (0-2 days between transfusions), and the elevation of the PLT, RBC and HCT vary, depending on the recipient’s condition and severity of the myelosuppression. With over 300 serological cross matched assessments performed, between donors and recipients, all evaluations resulted in cross matched compatibility. Our data suggest that donor to recipient cross match is not required for significantly immunosuppressed cynomolgus and rhesus monkeys. A blood transfusion volume of 7-14 mL/kg infused over 30-60 minutes was well tolerated in 99% of the animals. All blood transfusions that were not tolerated could be halted during the initial transfusion phase at a lower infusion rate, hence preventing complications and severe adverse reactions.